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Hydrolyzed Keratin

B
lead outcome
Topical (hair): overall as an anti-breakage…
grades vary by outcome ↓
Protein (not a peptide)
also called — hydrolysed keratin · keratin peptides · wool-derived keratin hydrolysate
hair conditioningfibre treatmentfilm former
In brief

A wool-derived protein hydrolysate used across hair care, and one of the better evidenced conditioning actives on the shelf, with a catch that undermines the ingredient list itself. Controlled fibre testing on chemically relaxed hair found that mid- and high-molecular-weight keratin peptides raised stiffness and cut breakage at both low and high humidity, with break stress up roughly 16 to 19 percent against untreated control. The lowest-weight fraction penetrated the cortex but delivered no comparable mechanical gain, and a free amino acid comparator left hair weaker than no treatment at all. Molecular weight is therefore the whole story, and it is precisely what an INCI list omits. Read the grade as reinforcement of fibre that already exists rather than repair of fibre that was damaged, because hair above the follicle cannot heal.

Legal standing, by region
International
Lawful cosmetic ingredient

Lawful for cosmetic use internationally, including the EU and UK under Regulation (EC) No 1223/2009 as retained, and the United States. It appears in no restricted or prohibited annex and carries no concentration limit.

Evidence, by outcome
How we grade →

An honest grade per outcome — drawn from the evidence, not any catalogue. Hype and undemonstrated marketing claims grade low.

OutcomeEvidence base · effectGrade
Topical (hair): overall as an anti-breakage treatment on chemically processed hair
Measured on fibres rather than as a blinded consumer outcome, on one hair type after sodium hydroxide relaxing. The benefit is mechanical reinforcement of existing fibre, not restoration, and it depends entirely on a property the ingredient list never discloses.
Controlled ex vivo study on chemically relaxed textured human hair comparing three keratin hydrolysates of defined molecular weight against an amino acid reference and untreated control, using single-fibre tensile testing, laser scanning micrometry, fluorescence microscopy and DSC (Malinauskyte 2021). · Mid- and high-molecular-weight keratin peptides raised Young's modulus and reduced breakage at both 20% and 80% relative humidity. Break stress rose significantly against the relaxed control, by roughly 18.6% for mid-weight and 16.3% for high-weight peptides at 20% relative humidity.
B
Topical (hair): cortex penetration by lower-molecular-weight fractions
Penetration and benefit do not track together. The smallest fraction reached the cortex but did not deliver the mechanical gain the larger fractions did, so depth alone is not the useful property.
Fluorescence microscopy and laser scanning micrometry in the same study, across hydrolysates of 221 Da, approximately 2577 Da and approximately 75 440 Da. · Low- and mid-weight fractions penetrated deep into the hair cortex. The high-weight fraction adsorbed onto the fibre surface and at most slightly entered the outer layers.
B
Marketing claim: repairs damaged hair, rebuilds the keratin structure
Hair above the follicle is non-living keratin and cannot heal. Reinforcement is the honest ceiling, and it washes out rather than accumulating.
Marketing claim. · No published evidence supports reconstruction of hair architecture. The measured effect is added reinforcement from deposited or adsorbed peptide, which is a different thing from rebuilding what bleach or relaxer destroyed.
D
Marketing claim: free amino acids as equivalent keratin building blocks
The building-block framing is intuitive and the data runs against it. Peptide chain length appears to matter more than the presence of amino acid material.
Direct comparison against L-leucine as an amino acid reference treatment in Malinauskyte 2021. · Hair treated with the free amino acid showed significantly lower break stress than the untreated relaxed control, performing worse than no treatment at all on that measure.
D
Safety (topical, rinse-off and leave-on hair use)
Protein hydrolysates carry a recognised, low sensitisation potential. Wool origin is worth knowing for anyone avoiding animal-derived ingredients.
Expert panel safety assessment of keratin and keratin-derived cosmetic ingredients (Burnett 2021), alongside long-standing use across hair categories. · Assessed as safe in current cosmetic practice at typical use concentrations in both rinse-off and leave-on hair formats.
Disclosure

Vallydia sells its own cosmetic serums, and some ingredients graded here belong to the same categories as those products. Grades are drawn from the published evidence by the method we publish, and applied to our own ingredients on the same terms — our copper-peptide serum is graded no more kindly than the peptides it competes with. We disclose the interest so you can weigh it.

Trade names shown here (for example Matrixyl, Argireline, Syn-Ake) are the property of their respective owners and are used only to identify the ingredient under discussion. Their appearance implies no affiliation with, or endorsement by, the proprietor.

Evidence changes

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Cosmetic claims boundary
✓ Allowed (appearance / feel)
  • helps reduce breakage in chemically processed hair
  • adds measurable stiffness and resilience to the hair fibre
  • conditions and smooths hair
  • well tolerated in rinse-off and leave-on hair formats
✕ Not allowed (medicinal)
  • repairs damaged hair
  • rebuilds keratin
  • restores hair structure
  • replaces lost protein
  • permanent repair

The medicinal-sounding science stays in the reference section; product copy speaks only to appearance/feel (Reg 655/2013). Different fields, never merged.

The honest part

The decisive property is molecular weight, and it is the one thing the label never tells you. Three materials with different behaviour, from 221 Da to over 75 000 Da, all appear on an ingredient list as the same two words. Two of the three reduced breakage; the smallest did not, and the free amino acid comparator was worse than no treatment. So the ingredient is real and the label is uninformative, which means the presence of "Hydrolyzed Keratin" in an INCI list predicts very little on its own.

Not the same as its namesake

Hydrolyzed Keratin is not interchangeable with Keratin Amino Acids or with free amino acid actives, and this card's grades do not extend to them. In the study underpinning those grades, the free amino acid reference performed worse than untreated control on break stress while the larger peptide fractions performed better. Chain length is the active variable, so a fully hydrolysed amino acid material should not inherit a peptide hydrolysate's evidence.

Identity

Hydrolyzed Keratin is keratin protein, most often sourced from wool, broken down by acid, alkaline or enzymatic hydrolysis into peptide fragments. It is not a single substance but a distribution of chain lengths, and commercial grades span an enormous range: materials characterised in the published fibre work ran from 221 Da through roughly 2577 Da to approximately 75 440 Da. All of them are declared identically on an ingredient list.

Its appeal is intuitive. Hair is keratin, so keratin is offered back to it. The evidence supports a narrower and more interesting story than that symmetry suggests.

Development & history

Protein hydrolysates have been used in hair care since the middle of the twentieth century, long predating the bond-building category and most of the actives that now command higher prices. Wheat and rice hydrolysates arrived alongside keratin, and each has been studied for penetration behaviour.

The molecular weight question was investigated systematically at TRI Princeton and published in 2021, testing defined fractions against both an untreated control and a free amino acid, on chemically relaxed textured hair. That design is what makes the data useful: it separates the effect of the peptide from the effect of simply wetting and treating the fibre, and it isolates chain length as a variable.

Mechanism (as proposed)

Two mechanisms are proposed and they operate at different depths.

Lower-molecular-weight peptides are small enough to diffuse into the cortex, where they are proposed to occupy and reinforce regions left porous by chemical processing, interacting with the keratin matrix through hydrogen bonding and electrostatic association rather than covalent attachment.

Higher-molecular-weight fractions largely cannot enter. They adsorb to the fibre surface and form a film, which is proposed to reduce friction, limit further damage and add measurable stiffness to the fibre as a whole.

Neither mechanism is reconstruction. Deposited peptide reinforces the fibre from inside or outside; it does not restore the cross-linked architecture that processing destroyed, and it is removed by washing. The observed mechanical gains are consistent with reinforcement, and the observation that the smallest fraction penetrated best while strengthening least is difficult to reconcile with any repair account.

Related reading

Our comparison of hydrolysed proteins in hair care sets keratin against wheat and rice hydrolysates. For the premium end of the same promise, see our examination of the bond-building category and what independent testing found there.

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Sources — 2 cited
01Malinauskyte E, Shrestha R, Cornwell PA, Gourion-Arsiquaud S, Hindley M. Penetration of different molecular weight hydrolysed keratins into hair fibres and their effects on the physical properties of textured hair. International Journal of Cosmetic Science. 2021;43(1):26-37. PMID 32946595.
02Burnett CL, Bergfeld WF, Belsito DV, Hill RA, Klaassen CD, Liebler DC, et al. Safety Assessment of Keratin and Keratin-Derived Ingredients as Used in Cosmetics. International Journal of Toxicology. 2021;40(2_suppl):36S-51S.
Updated 2026-08-28

Grades reflect the published evidence, not our interest. No dosing, reconstitution, or administration is published for research compounds — that restraint is deliberate.

Related compounds
Hydrolyzed Wheat ProteinC
Blend / combination
Hydrolyzed Rice ExtractB
Blend / combination
PanthenolB
Small molecule (non-peptide)
Amino Acids (Lysine & Arginine)C
Small molecule (non-peptide)
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This site provides neutral scientific reference and sells only products lawful in your region. Nothing here is medical advice, a recommendation, or an offer to supply unapproved medicines. No dosing or administration is published for research compounds. Cosmetic peptides per Regulation (EC) 1223/2009. Unapproved injectable peptides are neither sold nor advertised in the EU (Directive 2001/83/EC, Title VIII). © 2026 Vallydia.